Often asked: How To Disinfect Mushrooms Farming Without A Pressure Cooker?

Can you sterilize substrate without a pressure cooker?

You have to put the mushroom substrate in a hot water immersion tub. The temperature range up to 80 degrees Celsius. Leave the substrate in hot water for 60 to 90 minutes. Let it dry for 120 minutes.

Do you need a pressure cooker for mushrooms?

Every home mushroom cultivator should own a pressure cooker. It plays an essential role in the fight against contamination. They are used to sterilize grain spawn, agar, substrate, and more. Typically materials are cooked at 15 PSI for a given amount of time, which kills off any bacterial or spores.

How do you sterilize a mushroom substrate in a pressure cooker?

This will clean the PC for sure.

  1. Fill the pressure cooker with 1 inch or 2,5 cm of cold water.
  2. Place the rack in the PC.
  3. Close the pressure cooker.
  4. Get the PC to build up pressure by slowly heating it.
  5. When the pressure cooker is on 15 psi let it do is job for 45 minutes.

How do you sterilize jars without a pressure cooker?


  1. Wash each and every jar by hand or in the dishwasher.
  2. Allow the jars to dry.
  3. Arrange the glass canning jars in the oven on clean oven racks.
  4. Turn the oven on 225 degrees Fahrenheit.
  5. Allow the jars to cook in the oven for at least 20 minutes before using them.
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How do you sterilize substrates in a pressure cooker?

When sterilizing things like substrates, grain and agar mixes, you want to look for a pressure cooker that can maintain a pressure of 15 PSI, which allows for steam temperature of 121°C- hot enough to kill pretty much anything you need to worry about. Many people will ask if they can go with lower pressures.

Can you sterilize substrate?

There are a few ways that you can sterilize a substrate without using a pressure cooker. A process called tyndallization or fractional sterilization can also be used. This is accomplished by boiling jars or bags for a certain length of time over several days in a row.

How do you disinfect a mushroom substrate?

Just boil the grain in water for an hour, put it in clean quart jars, and then cook the whole thing in a pressure cooker for another hour at 15 psi. I soaked my cardboard substrate in hot water before inoculating, which I assume did a bit of sterilization at least.

Do mushrooms grow better in the dark?

In terms of the rate of growth at any given time, mushrooms do indeed grow better in the dark in the sense that they tend to grow faster in the dark than they do in the light.

How long does it take to sterilize jars in a pressure cooker?

Select either, set the pot to high pressure, adjust the timer to 15 minutes, and hit start. But if you have one of the latest models just press the “ sterilize ” button and let your instant pot do the magic.

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Can I use a pressure cooker to sterilize?

A pressure cooker works just as well as autoclaving, especially for small batches. Just like an autoclave, the pressure cooker chamber reaches temperatures high enough to kill bacteria and mold spores. Following the manufacturer’s instructions, seal the pressure cooker and heat it on a burner.

How much water do you put in a pressure cooker?

When you use a pressure cooker, you need to have enough liquid in the pot for it to come up to pressure and cook the food properly. The rule of liquids in pressure cooking is to always add at least 1 cup of liquid unless the recipe states otherwise. The liquid will help create enough steam to cook the meal.

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